clear milk blocking buffer Search Results


non fat milk blocking buffer  (Abmart Inc)
86
Abmart Inc non fat milk blocking buffer
Non Fat Milk Blocking Buffer, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/non fat milk blocking buffer/product/Abmart Inc
Average 86 stars, based on 1 article reviews
non fat milk blocking buffer - by Bioz Stars, 2026-05
86/100 stars
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5% milk blocking buffer  (Carl Roth GmbH)
90
Carl Roth GmbH 5% milk blocking buffer
5% Milk Blocking Buffer, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5% milk blocking buffer/product/Carl Roth GmbH
Average 90 stars, based on 1 article reviews
5% milk blocking buffer - by Bioz Stars, 2026-05
90/100 stars
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water with 5 % formic acid  (Carl Roth GmbH)
90
Carl Roth GmbH water with 5 % formic acid
Water With 5 % Formic Acid, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/water with 5 % formic acid/product/Carl Roth GmbH
Average 90 stars, based on 1 article reviews
water with 5 % formic acid - by Bioz Stars, 2026-05
90/100 stars
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milk blocking buffer  (Beijing CWBio)
90
Beijing CWBio milk blocking buffer
Milk Blocking Buffer, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/milk blocking buffer/product/Beijing CWBio
Average 90 stars, based on 1 article reviews
milk blocking buffer - by Bioz Stars, 2026-05
90/100 stars
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blocking buffer [milk diluent sera (kpl, seracare) and 0.1% tween in pbs]  (SeraCare Life Sciences)
90
SeraCare Life Sciences blocking buffer [milk diluent sera (kpl, seracare) and 0.1% tween in pbs]
Blocking Buffer [Milk Diluent Sera (Kpl, Seracare) And 0.1% Tween In Pbs], supplied by SeraCare Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blocking buffer [milk diluent sera (kpl, seracare) and 0.1% tween in pbs]/product/SeraCare Life Sciences
Average 90 stars, based on 1 article reviews
blocking buffer [milk diluent sera (kpl, seracare) and 0.1% tween in pbs] - by Bioz Stars, 2026-05
90/100 stars
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tsaye supplemented with 5% nacl (wt/vol)  (Applichem inc)
90
Applichem inc tsaye supplemented with 5% nacl (wt/vol)
Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. <t>Total</t> <t>culturable</t> (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on <t>TSAYE</t> supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.
Tsaye Supplemented With 5% Nacl (Wt/Vol), supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tsaye supplemented with 5% nacl (wt/vol)/product/Applichem inc
Average 90 stars, based on 1 article reviews
tsaye supplemented with 5% nacl (wt/vol) - by Bioz Stars, 2026-05
90/100 stars
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blocking buffer consisting of 5% skimmed milk powder in pbs marvel  (Cadbury Ltd)
90
Cadbury Ltd blocking buffer consisting of 5% skimmed milk powder in pbs marvel
Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. <t>Total</t> <t>culturable</t> (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on <t>TSAYE</t> supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.
Blocking Buffer Consisting Of 5% Skimmed Milk Powder In Pbs Marvel, supplied by Cadbury Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blocking buffer consisting of 5% skimmed milk powder in pbs marvel/product/Cadbury Ltd
Average 90 stars, based on 1 article reviews
blocking buffer consisting of 5% skimmed milk powder in pbs marvel - by Bioz Stars, 2026-05
90/100 stars
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blocking buffer 5% skim milk #8340  (Beijing Solarbio Science)
90
Beijing Solarbio Science blocking buffer 5% skim milk #8340
Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. <t>Total</t> <t>culturable</t> (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on <t>TSAYE</t> supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.
Blocking Buffer 5% Skim Milk #8340, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blocking buffer 5% skim milk #8340/product/Beijing Solarbio Science
Average 90 stars, based on 1 article reviews
blocking buffer 5% skim milk #8340 - by Bioz Stars, 2026-05
90/100 stars
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milk blocking buffer kirkegaard & perry  (SeraCare Life Sciences)
90
SeraCare Life Sciences milk blocking buffer kirkegaard & perry
Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. <t>Total</t> <t>culturable</t> (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on <t>TSAYE</t> supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.
Milk Blocking Buffer Kirkegaard & Perry, supplied by SeraCare Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/milk blocking buffer kirkegaard & perry/product/SeraCare Life Sciences
Average 90 stars, based on 1 article reviews
milk blocking buffer kirkegaard & perry - by Bioz Stars, 2026-05
90/100 stars
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assay buffer (5× milk diluent/block)  (SeraCare Life Sciences)
90
SeraCare Life Sciences assay buffer (5× milk diluent/block)
Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. <t>Total</t> <t>culturable</t> (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on <t>TSAYE</t> supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.
Assay Buffer (5× Milk Diluent/Block), supplied by SeraCare Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/assay buffer (5× milk diluent/block)/product/SeraCare Life Sciences
Average 90 stars, based on 1 article reviews
assay buffer (5× milk diluent/block) - by Bioz Stars, 2026-05
90/100 stars
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blocking buffer 10% non-fat milk in 1× tris-buffered saline (tbs)  (Corning Life Sciences)
90
Corning Life Sciences blocking buffer 10% non-fat milk in 1× tris-buffered saline (tbs)
Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. <t>Total</t> <t>culturable</t> (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on <t>TSAYE</t> supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.
Blocking Buffer 10% Non Fat Milk In 1× Tris Buffered Saline (Tbs), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blocking buffer 10% non-fat milk in 1× tris-buffered saline (tbs)/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
blocking buffer 10% non-fat milk in 1× tris-buffered saline (tbs) - by Bioz Stars, 2026-05
90/100 stars
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membrane blocking buffer 5% non-fat milk  (Labscientific)
90
Labscientific membrane blocking buffer 5% non-fat milk
Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. <t>Total</t> <t>culturable</t> (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on <t>TSAYE</t> supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.
Membrane Blocking Buffer 5% Non Fat Milk, supplied by Labscientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/membrane blocking buffer 5% non-fat milk/product/Labscientific
Average 90 stars, based on 1 article reviews
membrane blocking buffer 5% non-fat milk - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.

Journal: Microbiology Spectrum

Article Title: Listeria monocytogenes Sublethal Injury and Viable-but-Nonculturable State Induced by Acidic Conditions and Disinfectants

doi: 10.1128/Spectrum.01377-21

Figure Lengend Snippet: Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.

Article Snippet: To differentiate the injured subpopulation from the total culturable population, growth in TSAYE supplemented with 5% NaCl (wt/vol) (AppliChem) was compared to growth in TSAYE with 0.5% NaCl.

Techniques: Incubation

Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 30, 60, 300, 900, or 1,800 s under the following conditions: (A) PAA, 0.5 ppm, at 20°C, (B) PAA, 5 ppm, at 20°C, (C) PAA, 10 ppm, at 20°C, (D) PAA, 0.5 ppm, at 4°C, (E) PAA, 5 ppm, at 4°C, (F) PAA, 10 ppm, at 4°C, (G) SH, 0.5 ppm, at 20°C, (H) SH, 5 ppm, at 20°C, (I) SH, 10 ppm, at 20°C, (J) SH, 0.5 ppm, at 4°C, (K) SH, 5 ppm, at 4°C, and (L) SH, 10 ppm, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). *, P < 0.1; **, P < 0.05; ***, P < 0.01; n.s., not significant.

Journal: Microbiology Spectrum

Article Title: Listeria monocytogenes Sublethal Injury and Viable-but-Nonculturable State Induced by Acidic Conditions and Disinfectants

doi: 10.1128/Spectrum.01377-21

Figure Lengend Snippet: Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 30, 60, 300, 900, or 1,800 s under the following conditions: (A) PAA, 0.5 ppm, at 20°C, (B) PAA, 5 ppm, at 20°C, (C) PAA, 10 ppm, at 20°C, (D) PAA, 0.5 ppm, at 4°C, (E) PAA, 5 ppm, at 4°C, (F) PAA, 10 ppm, at 4°C, (G) SH, 0.5 ppm, at 20°C, (H) SH, 5 ppm, at 20°C, (I) SH, 10 ppm, at 20°C, (J) SH, 0.5 ppm, at 4°C, (K) SH, 5 ppm, at 4°C, and (L) SH, 10 ppm, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). *, P < 0.1; **, P < 0.05; ***, P < 0.01; n.s., not significant.

Article Snippet: To differentiate the injured subpopulation from the total culturable population, growth in TSAYE supplemented with 5% NaCl (wt/vol) (AppliChem) was compared to growth in TSAYE with 0.5% NaCl.

Techniques: Incubation

Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 30, 60, 300, 900, or 1,800 s under the following conditions: (A) PAA, 20 ppm, at 20°C, (B) PAA, 30 ppm, at 20°C, (C) PAA, 40 ppm, at 20°C, (D) PAA, 20 ppm, at 4°C, (E) PAA, 30 ppm, at 4°C, and (F) PAA, 40 ppm, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). *, P < 0.1; **, P < 0.05; n.s., not significant.

Journal: Microbiology Spectrum

Article Title: Listeria monocytogenes Sublethal Injury and Viable-but-Nonculturable State Induced by Acidic Conditions and Disinfectants

doi: 10.1128/Spectrum.01377-21

Figure Lengend Snippet: Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 30, 60, 300, 900, or 1,800 s under the following conditions: (A) PAA, 20 ppm, at 20°C, (B) PAA, 30 ppm, at 20°C, (C) PAA, 40 ppm, at 20°C, (D) PAA, 20 ppm, at 4°C, (E) PAA, 30 ppm, at 4°C, and (F) PAA, 40 ppm, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). *, P < 0.1; **, P < 0.05; n.s., not significant.

Article Snippet: To differentiate the injured subpopulation from the total culturable population, growth in TSAYE supplemented with 5% NaCl (wt/vol) (AppliChem) was compared to growth in TSAYE with 0.5% NaCl.

Techniques: Incubation

Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, or 3 h at 20°C in Ringer’s solution with 20 ppm (A), 30 ppm (C), or 40 ppm (E) PAA. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from one experiment with six independent samples ( n = 6). Statistically significant differences ( P < 0.05) are indicated with different uppercase and lowercase letters for TSAYE and TSAYE+5%NaCl, respectively. The dotted line represents the limit of detection (LOD). ***, P < 0.01; n.s., not significant. Each time point relates to several images acquired at different fields of view (FOV) of the same sample. Enumeration results (%) for cells exhibiting fluorescence (CFDA + /PI − , green; CFDA − /PI + , red; CFDA + /PI + , orange; or CFDA − /PI − , black), considering several images of different fields of view (FOV) at each time point after treatment with PAA at 20 ppm (0 h, 5 FOV; 1 h, 4 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (B), 30 ppm (0 h, 4 FOV; 1 h, 5 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (D), and 40 ppm (0 h, 4 FOV; 1 h, 5 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (F).

Journal: Microbiology Spectrum

Article Title: Listeria monocytogenes Sublethal Injury and Viable-but-Nonculturable State Induced by Acidic Conditions and Disinfectants

doi: 10.1128/Spectrum.01377-21

Figure Lengend Snippet: Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, or 3 h at 20°C in Ringer’s solution with 20 ppm (A), 30 ppm (C), or 40 ppm (E) PAA. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from one experiment with six independent samples ( n = 6). Statistically significant differences ( P < 0.05) are indicated with different uppercase and lowercase letters for TSAYE and TSAYE+5%NaCl, respectively. The dotted line represents the limit of detection (LOD). ***, P < 0.01; n.s., not significant. Each time point relates to several images acquired at different fields of view (FOV) of the same sample. Enumeration results (%) for cells exhibiting fluorescence (CFDA + /PI − , green; CFDA − /PI + , red; CFDA + /PI + , orange; or CFDA − /PI − , black), considering several images of different fields of view (FOV) at each time point after treatment with PAA at 20 ppm (0 h, 5 FOV; 1 h, 4 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (B), 30 ppm (0 h, 4 FOV; 1 h, 5 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (D), and 40 ppm (0 h, 4 FOV; 1 h, 5 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (F).

Article Snippet: To differentiate the injured subpopulation from the total culturable population, growth in TSAYE supplemented with 5% NaCl (wt/vol) (AppliChem) was compared to growth in TSAYE with 0.5% NaCl.

Techniques: Incubation, Fluorescence